The effects of three different adjuvants – mineral oil, Montanide^TMISA 70M VG and Montanide ISA 206 VG – were evaluated on reverse genetics H5N3 avian influenza virus cell-cultured vaccine by Chun G. Liu of Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences and co-authors there and at Guizhou University. Their paper has been published in Virology Journal.

The immune results of specific pathogen-free (SPF) chickens after challenge with highly pathogenic avian influenza (HPAI) virus demonstrated that mineral oil adjuvant group and 70M adjuvant group provided 100 per cent protection efficiency but the 206 adjuvant group provided only 40 per cent protection. Statistical analysis indicated that there were no significant differences in the protection effects of mineral oil adjuvant group and the 70M adjuvant but the differences with the 206 adjuvant group were significant.

All three adjuvants induced high titres of antibody after immunising SPF ducks but there was no significant difference between them.

Liu and co-authors concluded that the immunisation effect of 70M adjuvant group on SPF chickens were the best and showed significant differences with optimised 70Mi Montanide eight series adjuvants groups.

These results suggest that 70M adjuvant could be a novel adjuvant for preparing avian influenza vaccine, the researchers added.

Reference

Liu C.G., M. Liu, F. Liu, D.F. Liu, Y. Zhang, W.Q. Pan, H. Chen, C.H. Wan, E.C. Sun, H.T. Li and W.H. Xiang. 2011. Evaluation of several adjuvants in avian influenza vaccine to chickens and ducks. Virology Journal, 8:321 doi:10.1186/1743-422X-8-321

Further Reading

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Further Reading

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You can visit the Avian Flu page by clicking here.

September 2011