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Researchers Can Detect Parvovirus in Geese
CHINA - Yang at Chongqing Academy of Animal Science and co-authors have published a paper in the latest issue of Virology Journal describing their success in developing a PCR method to detect goose parvovirus in vivo.Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese, explain Yang and co-workers. Currently, it severely affects geese production worldwide.
The objective of their study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo.
Results
The detection limit of the assay was 2.8 × 101 standard DNA copies, with a sensitivity of three logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of variation.
Conclusion
The high sensitivity, specificity, simplicity and reproducibility of the GPV fluorogenic PCR assay, combined with a high throughput, make this method suitable for a broad spectrum of GPV etiology-related applications, conclude Yang et al.
Reference
Yang J-L., A-C. Cheng, M-S. Wang, K-C. Pan, M. Li, Y-F. Guo, C-F. Li, D-K. Zhu and X-Y. Chen. 2009. Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo. Virology Journal 2009, 6:142 doi:10.1186/1743-422X-6-142
Further Reading
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- | You can view the full report by clicking here. |
Further Reading
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- | Find out more information on goose parvovirus (Derzsy's disease) by clicking here. |
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